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A game changer in sample preparation for plasma proteomics

Working with plasma samples can be challenging due to the blood complexity itself and the high-dynamic range of proteins within the biological samples. High-abundance proteins such as albumin and immunoglobulins make up a significant portion of the specimen and limit access to low-abundance proteins, which are more likely to represent relevant biomarkers in different applications, such as disease diagnosis and drug development. Dr. Hyonson Hwang, Research Scientist at Caris Life Sciences, faced the high-dynamic range issue with plasma while working with human plasma for proteomic mass spec analysis. She turned to PreOmics solutions and was impressed to find that the iST technology not only outperforms standard methods but also provides user-friendly kits and protocols, ensuring consistently reproducible results.

Here's what she had to say:

"When I first started working with human plasma for proteomic mass spec analysis, I quickly ran into the well-known dynamic range issue with plasma. I tried several different standard sample preparation protocols but struggled to get adequate results. I then came across PreOmics® and their iST-BCT kit for plasma proteomics. I was pleasantly surprised that I could identify as many plasma proteins as I did by using such a relatively low amount of starting material (<2 µL of human plasma) in comparison to standard protocols, such as depletion columns or enrichment of low abundant plasma proteins. It consistently outperforms any standard methods I used in the market, and I fell in love with it due to the following reasons: overall ease of use, the very helpful color-coordinated reagents and protocol steps, and the consistently reproducible results. I’ve since tested a variety of PreOmics®' other products, the BeatBox®, iST kit, and ENRICH kit, and have been equally impressed. Instead of spending time trying to optimize mass spec sample preparation protocols like before, using PreOmics®’ products, I have more time for data analysis and interpretation, experimental design, and development of mass spec data collection methods."