Smile! High-throughput protein sample preparation automated

Automated proteomics sample prep instrument - compatible with PreOmics® iST and iST-BCT workflows

The challenge:

Manually preparing samples for LC-MS analysis can be challenging due to the intensive hands-on time required and the lack of reproducibility. Achieving a robust, reliable, and reproducible workflow while starting from a wide range of biologically complex sample matrices is essential to unlocking the full potential of proteomics analysis.

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The solution:

The Automated ProteinPrep 96 (APP96^TM) is a versatile, user-friendly automated micro chromatography-based platform that uses packed resin-bed cartridges (PreOmics Puritication tips, POPtips^TM), precision-flow control, and PreOmics®’ iST technology to carry out a wide variety of sample preparation workflows for LC-MS analysis. It can prepare from 1 to 96 samples in parallel, ensuring standardization and reducing the hands-on time to 10 min!

Apply! Simple, streamlined workflows

1. Samples

Samples should be in solution or cell pellets.

2. Automated sample preparation

From lyse to eluted peptides.

3. LC-MS analysis

Inject and analyze to see improved peptide identities.

4. Data analysis

Process your data to see improved peptide identities.

Results

PreOmics^®'

APP96

values

Smile

Improved sample quality

High reproducibility: CV = 5-8% for intra-day and CV = 17% for inter-day repeatability
Working range: 1-100 µg
Can prepare up to 96 LC-MS samples in parallel
Fully compatible with PreOmics®’ iST and iST-BCT technology
Compact: Only 85 cm (33.4 inches) in length
Installation and training included
No external computer needed

Relax

Sample preparation fully automated

True walk-away automation for label-free workflows
Unsupervised overnight sample preparation option
Reduces manual processing steps (only 10 mins of hands-on time)
Pre-loaded iST scripts (label-free applications)
Reduces plastic waste by saving hundreds of disposable tips per day

Realize

Cleaner peptides ready for analysis

Dual clean-up step removes hydrophilic and hydrophobic contaminants
Reduce mass spectrometry downtime
Precise flow rate control ensures the highest performance during washes and elution steps
Tip-based cleanup and elution: no centrifuge or positive pressure needed
Onboard heater shaker