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The challenge:
How do you automate sample preparation of very dirty samples or where harsh denaturation reagents are needed? Typically these samples cause issues with proteomic sample preparation as the reagents can adversely impact digestion and subsequent LC-MS analysis.
The solution:
Use the combined advantages of SP3® and iST! Together, PreOmics® and Tecan®* have developed an automated LC-MS sample preparation workflow, combining the ability to wash and digest samples when bound to the SP3® beads and then do the clean-up step. This application meets the demands of efficient, robust proteomics sample preparation for difficult samples for LC-MS analysis with the bonus that an automated workflow helps labs decrease the hands-on time and produce high quality samples for LC-MS analysis!
*“Tecan” is a registered Trademark of a third party
Apply! Simple, streamlined workflows
1. Adding samples
Samples should be liquid, homogenates or a soft pellet.
2. SP3-iST kit set up
Prepare the SP3 beads and the DIGEST and add all reagents to the Tecan instruments.
3. Sample preparation
Wash, lyse and digest samples while bound to SP3 beads on the Tecan Freedom EVO®
4. Sample clean up
Samples are automatically transferred to the clean-up plate and processed on the Tecan Resolvex® A200.
5. MS and data analysis
Analyze samples by LC-MS and process the data.
Results
Additional data can be found in the Tecan® SP3-iST application note. The numbers of PSMs and IDs for both peptides and proteins were very consistent for all samples, regardless of protein input, as well as between the manual and the automated protocols. Approximately 27,370 peptides and 2,960 proteins were identified in all samples, in agreement with the expected performance of the nanoLC-MS measurements conducted. This demonstrates the high efficiency, quality and robustness of the automated SP3-iST workflow for the entire range of tested input amounts (15-50 μg).
PreOmics®'
Tecan
values
Smile
Improved sample quality
- Working range: 1-100 µg
- Good consistency between manual and automated workflows
- Excellent missed cleavage rate
Relax
Challenging sample preparation automated
- Benefit from combined SP3® and iST technology
- Set up and walk away, hands-free LC-MS sample preparation
- Fully flexible, process 1 to 96 samples
Realize
Cleaner peptides ready for analysis
- Standardized workflow with high data reproducibility
- Dual clean up step removes hydrophilic and hydrophobic contaminants
- Achieve ready-to-measure peptides
Tecan
Tecan
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Instrument
Instrument
Application for high throughput proteomic sample preparation for LC-MS analyses using iST and SP3®
Tecan
Request quote
Instrument
Instrument
Application for high throughput proteomic sample preparation for LC-MS analyses using iST and SP3®
Tecan
Request quote
Instrument
Instrument
Application for high throughput proteomic sample preparation for LC-MS analyses using iST and SP3®
Request quote
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The PreOmics® products displayed are for visual representation only and may differ from those received
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ENRICH-iST | Simplified, automated sample preparation for plasma proteomics | Tecan – Resolvex® i300 on Fluent®
ENRICH-iST
TECAN
ENRICH-iST | Simplified, automated sample preparation for plasma proteomics | Tecan – Resolvex® i300 on Fluent®
ENRICH-iST | High throughput sample prep for extensive plasma proteomics | Tecan – Resolvex® A200 on Fluent®
ENRICH-iST
TECAN
ENRICH-iST | High throughput sample prep for extensive plasma proteomics | Tecan – Resolvex® A200 on Fluent®
iST-PSI | Automation of the iST-PSI PreOmics workflow on Tecan`s Freedom EVO® 100 and Resolvex® A200 | Tecan
iST-PSI
TECAN
iST-PSI | Automation of the iST-PSI PreOmics workflow on Tecan`s Freedom EVO® 100 and Resolvex® A200 | Tecan
We currently don't have resource for this, but feel free to check the other tabs
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