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en
Cell lines
Model organisms
Plant tissues
Biological fluids
Immunoprecipitations
Mammalian tissues
FFPE tissues
Cell lines
Cell lines and primary cells can be directly processed with PreOmics® sample preparation technology for a wide range of applications. Typical experiments may include protein-binding interactions, pathway elucidation, bottom-up proteomics and semi-targeted work. For example when testing cell therapies assessing the up-regulation or down-regulation of the target protein is essential.
The iST/iST-NHS kits are suitable for use in research and regulated environments. The kits contain all you need for your peptide or protein sample preparation and are suitable for use by all members of your lab, no specialist experience is needed. The flexible formats of single samples or racked in to 96 well holders allow the kits to be used manually or with a range of automation options. The cartridges can be processed by centrifugation or using positive pressure manifolds, again giving options for all labs. Dedicated resources are shown below and our technical support team would be happy to help with advice.
Please always ensure the total protein present is within the 1 – 100 µg range. In our labs we have established that working to a set OD or cell count is more reproducible than a total protein concentration assay, initially you may need to make measurements of total protein vs OD or cell count until you have a good understanding of the relationship. Guidelines are given below dependent on the starting state of the cell line material. Cell lines and primary cells can be directly processed with PreOmics® sample preparation technology for a wide range of applications. Typical experiments may include protein-binding interactions, pathway elucidation, bottom-up proteomics and semi-targeted work. For example when testing cell therapies assessing the up-regulation or down-regulation of the target protein is essential.
Cell lines and primary cells can be directly processed with PreOmics® sample preparation technology for a wide range of applications. Typical experiments may include protein-binding interactions, pathway elucidation, bottom-up proteomics and semi-targeted work. For example when testing cell therapies assessing the up-regulation or down-regulation of the target protein is essential.
The iST/iST-NHS kits are suitable for use in research and regulated environments. The kits contain all you need for your peptide or protein sample preparation and are suitable for use by all members of your lab, no specialist experience is needed. The flexible formats of single samples or racked in to 96 well holders allow the kits to be used manually or with a range of automation options. The cartridges can be processed by centrifugation or using positive pressure manifolds, again giving options for all labs. Dedicated resources are shown below and our technical support team would be happy to help with advice.
Please always ensure the total protein present is within the 1 – 100 µg range. In our labs we have established that working to a set OD or cell count is more reproducible than a total protein concentration assay, initially you may need to make measurements of total protein vs OD or cell count until you have a good understanding of the relationship. Guidelines are given below dependent on the starting state of the cell line material. Cell lines and primary cells can be directly processed with PreOmics® sample preparation technology for a wide range of applications. Typical experiments may include protein-binding interactions, pathway elucidation, bottom-up proteomics and semi-targeted work. For example when testing cell therapies assessing the up-regulation or down-regulation of the target protein is essential.
Resources
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