Smile! Plasma and serum sample preparation simplified

Accessing low-abundant proteins of interest in plasma and serum samples is challenging. The high dynamic range in protein concentrations and the heterogeneity and complexity pose significant obstacles for LC-MS-based proteomics, limiting access to full proteome information. In consequence, results lack robustness and reproducibility, traditional workflows are complex and time-consuming and low-abundant proteins are masked by high-abundant proteins. Thus, the impracticality of automation, the lack of flexibility and the inability to produce unbiased results impede the application for large sample cohorts.

The ENRICH-iST kit provides an easy-to-use, yet robust solution to the dynamic range challenge of plasma and serum samples. Low-abundant proteins are enriched onto paramagnetic beads while analytical depth and proteome coverage are conserved. With scalable throughput, exceptional reproducibility, and low CVs, the process takes only 5h from sample to pure peptides. ENRICH-iST is compatible with human samples and other mammalian species like mouse, rat, pig, or dog. Thanks to the simple protocol and compatibility with established lab automation systems the processing of large sample cohorts becomes an easy to achieve reality.