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Smile! Protein and peptide sample preparation automated

Application for high throughput proteomic sample preparation for LC-MS analysis

The challenge:

How do you improve reproducibility and hands on time savings in proteomics sample preparation for LC-MS analysis when you have already addressed the challenges of time, sample contamination and loss?

The solution:

Automate! Together, PreOmics® and Hamilton®* have developed a completely automated high throughput LC-MS sample preparation workflow, combining the Hamilton® liquid handling technology with the PreOmics® iST-PSI workflow. To meet the demands of high-throughput proteomics sample preparation, this fully automated, efficient and reproducible workflow helps decrease the processing time to about 4 hours and produces high quality samples for LC-MS analysis!

*“Hamilton” is a registered Trademark of a third party

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Apply! Simple, streamlined workflows

1. Adding samples

Samples should be liquid, homogenates or a soft pellet.

2. iST-PSI kit setup

Set up the iST-PSI consumables on the HamiltonMicrolab® STAR V Liquid Handling System®.

3. Sample preparation

Run pre-defined protocols in consultation with your automation specialist.

4. MS analysis

Analyze samples by LC-MS.

5. Data analysis

Process data to see improved peptide identities.

Results

**Figure 1:** Inter-day reproducibility and comparison to manual processing.(A and B) 8 aliquots of manually prepared *P. pastoris* protein extracts(50 µg each) were processed on two different days, and 4 additional samples were digested with the manual procedure.(C and D) 8 samples of commercially available human plasma (each~70 µg; Sigma-Aldrich, P9523) were processed on day 1 and 2.

**Figure 2:** 96 sample run – 48 aliquots of manually extracted *P. pastoris* proteins were processed together with 48 samples of commercially available human plasma.

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More detailed explanation about these results can be found on the Hamilton® application note.

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PreOmics^®'

Hamilton

values

Smile

Improved sample quality

High reproducibility: Pearson correlation > 0.93
Working range: 1-100 µg
Increased peptide and protein identifications

Relax

Fully automated high throughput sample preparation

Set up and walk away, hands-free LC-MS sample preparation
Fully flexible, process 1 to 96 sample
Minimal tip usage

Realize

Cleaner peptides ready for analysis

Dual clean up step removes hydrophilic and hydrophobic contaminants
Achieve ready-to-measure peptides
Reduce mass spectrometry downtime
Standardized workflow with high data reproducibility and process safety

PepSep® Columns & Emitters

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Application for high throughput proteomic sample preparation for LC-MS analysis

Hamilton

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Instrument

Application for high throughput proteomic sample preparation for LC-MS analysis

Hamilton

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Instrument

Application for high throughput proteomic sample preparation for LC-MS analysis

PepSep® Classics* – Performance Meets Simplicity

Versatile columns for standard and advanced proteomics workflows.

50 cm × 75 µm × 1.5 µm → Spectral Library Generation | Art.-Nr. 1895846
25 cm × 75 µm × 1.5 µm → Sensitivity | Art.-Nr. 1893484
25 cm × 150 µm × 1.5 µm → Versatility | Art.-Nr. 1893476
15 cm × 150 µm × 1.5 µm → Throughput | Art.-Nr. 1893474
8 cm × 150 µm × 1.5 µm → Speed | Art.-Nr. 1893470

* additional column dimensions available. The full list of dimensions can be found in the brochure

PepSep® Advanced – Next Level Sensitivity

For the highest performance and maximum peptide coverage.

25 cm × 75 µm × 1.5 µm → New gold standard | Art.-Nr. 1919826

CaptiveSpray 2 Emitters

Plug-and-play emitters for hassle-free CaptiveSpray setup. Two variants, separate from columns for easy replacement and longer LC-MS system life with peak performance (Pack of 2 pieces).