Smile! Immunoprecipitation sample preparation simplified

Fast, reliable, preparation from immunoprecipitated samples ready for mass spectrometry

The challenge:

Integrating immunoprecipitation with multi-step methods of proteomics sample preparation for mass spectrometry analysis has inherent challenges of time, contamination and sample loss. Preparing labeled samples over extended period with a complex method leads to variation in results especially when working with high sample throughput and small starting amounts of input material.

The solution:

The PreOmics® / ChromoTek iST-Trap Kit enables the fast and efficient immunoprecipitation of GFP-Fusion proteins and the interaction factors followed by the innovative sample preparation for mass spectrometry based proteomics. All in less than 4 hours.

Apply! Simple, streamlined workflows

1. Sample immunoprecipitation

Samples should be liquid, add GFP-Trap_A and incubate at 4°C for 60 min.

2. Denature, reduce and alkylate

Add LYSE reagent, and incubate for 10 mins at 60°C.

3. Digest

Cool, add DIGEST reagent, incubate for 60 mins at 37°C.

4. Purify

Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.

5. MS analysis

Inject and analyze to see improved peptide identities.

Results

Time flies with PreOmics® iST-Trap sample preparation

While standard proteomics sample preparation takes up to 44 hours – the same time as flying around the world non-stop, PreOmics® kits take the same time as flying from our Munich HQ to London or from Boston to Chicago. Increase your sample throughput, improve quality and produce reliable data. It’s as easy as i-S-T!