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Optimizing plasma and serum sample preparation for deeper, high-throughput proteome profiling
Fast and efficient sample preparation workflows targeting low-abundance proteins in plasma, serum, CSF, and other high dynamic range samples for application in academic and biopharma research
The challenge
Accessing low-abundance proteins in plasma, serum, CSF and other high dynamic range samples for LC-MS-based proteomic analysis is very challenging due to the complexity and high dynamic range of proteins within these biological samples. High-abundance proteins such as e.g. albumin and immunoglobulins in plasma or serum make up a significant portion of the specimen and limit access to low-abundance proteins, which are more likely to represent relevant biomarkers in different applications.
The solution
ENRICH is an all-in-one solution involving the bead-based enrichment of low-abundance proteins, followed by digestion and purification on paramagnetic beads using the PreOmics® iST method. ENRICH technology is compatible with human samples and other mammalian species. With scalable throughput and exceptional reproducibility, the sample preparation takes only 5 hours from raw samples to pure peptides, and the analysis of large cohorts is possible with established lab automation systems suitable for magnetic bead processing. Starting at $100 per sample, ENRICH technology is democratizing access to deep, unbiased plasma proteome analysis for diverse research needs.
ENRICH-iST is a flexible all-in-one solution to prepare plasma, serum, CSF and other high dynamic range samples for proteomic analysis. It is based on the enrichment of low-abundance proteins onto paramagnetic beads and is compatible with human and other mammalian samples (e.g., mouse, rat, dog, pig, non-human-primate). The scalable throughput, exceptional reproducibility and a ultra-fast protocol from raw samples to pure peptides make ENRICH-iST a reliable tool for verifying and discovering biomarkers in large cohorts.
Results
Deeper proteome coverage compared to antibody-based depletion
According to the protein concentration from the Human Protein Atlas for plasma, ENRICH-iST offers superior analytical depth and proteome coverage.
Streamlined workflow for validation of known and novel biomarkers
ENRICH-iST identified 16 additional biomarkers in comparison to neat plasma.
Automatable, standardized workflow and higher reproducibility for reliable biological insights
ENRICH-iST shows a robust workflow with low technical variability: Protein CV of MaxLFQs < 20 % and equal quantitative performance between manual and automated sample preparation.
Coming soon in 2025 – ENRICHplus: Discover our upcoming kits for deep, high-throughput, fast, automatable, and affordable plasma proteome access with precise quantification. PreOmics’ ENRICHplus kits increase protein identifications up to 7-fold with excellent reproducibility (CV 11%). In a study of 12 participants, ENRICHplus identified 5,800 protein groups in human EDTA plasma*. This cost-effective solution supports automated workflows and, when combined with Bruker’s timsTOF HT instruments and Biognosys’ Spectronaut® 19, creates a powerful yet accessible plasma analysis platform.
*Recommended for human EDTA plasma. Other anticoagulants or biofluids might not work. Please consult with our technical experts.
*Recommended for human EDTA plasma. Other anticoagulants or biofluids might not work. Please consult with our technical experts.
Results
Fig. 1: (A) Streamlined plasma workflow combining PreOmics' ENRICHplus sample preparation, Bruker's timsTOF HT mass spectrometers and Biognosys Spectronaut® 19 software package. (B) Protein identifications of neat and ENRICHplus-processed human EDTA plasma samples (pool of 6 healthy donors, 6 colorectal cancer patients).
"We tested several options for biomarker discovery on plasma samples, but many were long and tedious. With the novel ENRICH technology, we have a fast, efficient, and reliable enrichment workflow to get deeper into the proteome from human plasma and serum. We use the ENRICH-iST 96x kits and are delighted by the reproducibility and ease of the kit. We really enjoyed working with the PreOmics team, who was always there to support us. We would strongly recommend the kit to other plasma and serum users."
Dr. Catherine Nury
Manager Proteomics, Philip Morris International, Switzerland
“ENRICHplus is changing the way we do plasma proteomics. We never before achieved such depth with a single run of plasma, now enabling us to detect tissue leakage proteins and other low abundant potential biomarkers for cancer and neurodegenerative diseases. ENRICHplus is a complete, efficient and game changing platform for deep plasma proteomics."
Roman Fischer
Associate Professor and Head of Discovery Proteomics Facility, University of Oxford
Key benefits of the ENRICH-iST technology
Enhanced proteome depth
Streamlined enrichment of low-abundance plasma proteins coupled with iST proteomic sample preparation technology for enhanced plasma proteome depth
Fast and reliable results
A fast, easy-to-use, and standardized protocol for high reproducibility and less hands-on time
Versatile technology
Species-independent and flexible technology compatible with plasma, serum, CSF, and other high dynamic range samples of different mammalian species
Automation friendly
Automatable high-throughput processing of up to 96 samples in parallel in just 5h
Low input samples
Optimized technique to work with low input starting material (20-80 μL) for large-scale proteomic studies
