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Plan! Use PreOmics®' products for your research
Resources for working with biological fluids
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Cell lines
Model organisms
Plant tissues
Biological fluids
Immunoprecipitations
Mammalian tissues
FFPE tissues
Biological fluids
Biological fluids may be the starting point for a wide range of experimental investigations from discovery protein interaction studies, preclinical toxicology assessments, through to large clinical trial biomarker measurements. For example monitoring biomarkers in saliva is growing in interest because of the ease of collection from patients. Biofluids including plasma, serum, saliva, urine, CSF, synovia and others are compatible for processing with our iST or iST-BCT and iST-NHS kits.
The PreOmics® kits are suitable for use in research and regulated environments. The kits contain all you need for your peptide or protein sample preparation and are suitable for use by all members of your lab, no specialist experience is needed. The flexible formats of single samples or racked in to 96 well holders allow the kits to be used manually or with a range of automation options.
The catridges can be processed by centrifugation or using positive pressure manifolds, again giving options for all labs. Dedicated resources are shown below and our technical support team would be happy to help with advice.
Outline processing considerations: Samples with volumes of 1-11 µL can be directly mixed with appropriate LYSE buffer as described in the regular protocols. Volumes higher than 11 µL either require prior precipitation, concentration or mixing with our higher concentrated lysis buffers. Please ensure that the total protein present is within the range 1 – 100 µg.
Biological fluids may be the starting point for a wide range of experimental investigations from discovery protein interaction studies, preclinical toxicology assessments, through to large clinical trial biomarker measurements. For example monitoring biomarkers in saliva is growing in interest because of the ease of collection from patients. Biofluids including plasma, serum, saliva, urine, CSF, synovia and others are compatible for processing with our iST or iST-BCT and iST-NHS kits.
The PreOmics® kits are suitable for use in research and regulated environments. The kits contain all you need for your peptide or protein sample preparation and are suitable for use by all members of your lab, no specialist experience is needed. The flexible formats of single samples or racked in to 96 well holders allow the kits to be used manually or with a range of automation options.
The catridges can be processed by centrifugation or using positive pressure manifolds, again giving options for all labs. Dedicated resources are shown below and our technical support team would be happy to help with advice.
Outline processing considerations: Samples with volumes of 1-11 µL can be directly mixed with appropriate LYSE buffer as described in the regular protocols. Volumes higher than 11 µL either require prior precipitation, concentration or mixing with our higher concentrated lysis buffers. Please ensure that the total protein present is within the range 1 – 100 µg.
Resources
iST-Fractionation | Novel cartridge-based peptide fractionation enables highly reproducible in-depth proteome coverage
iST-Fractionation | Novel cartridge-based peptide fractionation enables highly reproducible in-depth proteome coverage
