Take a leap forward in tissue and cell preparation efficiency

High-throughput homogenization technique for fast and reproducible release of proteins for all your research applications

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The challenge

Tissue homogenates and cell lysates are the starting point for a wide variety of downstream applications in genomics and proteomics. The extracted biomolecules are further characterized with methods, such as genotyping, immunoassays, and mass spectrometry, in order to identify biomarkers or understand cellular pathways.

Particularly proteomics requires thoroughly homogenized samples to obtain high protein yields even from small inputs like needle biopsies for sensitive and quantitative analyses. Furthermore, standardization, reproducibility, and parallelization are key to the processing of large numbers of samples in high-throughput environments. However, scientists will require a homogenization method that allows for high flexibility and versatility to cover a broad range of projects.

The solution

BeatBox® is a dedicated system for convenient, semi-automated sample homogenization of many tissue types and cells. Flexible regarding input amounts from 1-50 mg, it consistently delivers reproducible results even for challenging downstream analyses like LC-MS-based proteomics with scalable throughput from 1-96 samples.

Results

Compatible sample types

BeatBox® technology has been used successfully to extract proteins by homogenization from a wide variety of biological materials with varying textures and rigidity.

Comparison of protein yields from small amounts of mouse tissues

1-2 mg of four different mouse tissues were homogenized using the BeatBox® and two other methods. Results clearly show the same or increased protein yield using BeatBox® comparatively with good reproducibility evidenced by the %CV results.

Comparison of protein yields from three cell types

HEK293 (4E5 cells), E. coli (8.4E7 cells) and S. cerevisiae (4.4E6 cells) were homogenized using the BeatBox® and sonication. Data clearly shows a significantly decreased %CV across all three cell types using the BeatBox.

"Busy MS facilities such as our own rely on efficient, fast, and robust tissue preparation. After a very positive evaluation of the new scalable BeatBox® solution from PreOmics®, we immediately adopted the workflow. Having such a quiet, compact tissue preparation solution on our bench day by day can only further serve to increase our efficiency. Furthermore, BeatBox® allows us to improve our MS facility utilization day by day. Our partnership with PreOmics® remains strong and one where we can exchange ideas and bring long-term outcomes together."
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Prof. Karl Mechtler

Head of Proteomics Tech Hub at VBCF, Vienna, Austria

"Beatbox has become an indispensable tool in our laboratory at the Max Planck Institute of Biochemistry, Munich. It is our go-to instrument for tissue homogenization, whether dealing with needle biopsy samples from clinics or bulk tissues. Its compatibility with 96-well microtiter plates seamlessly integrates the homogenization process with the iST proteomics sample preparation kit, right through to LC-MS sample injection."

Prof. Dr. Matthias Mann,

Director Research Department "Proteomics and Signal Transduction" at the Max Planck Institute of Biochemistry:

"Having such a quiet, compact tissue preparation solution on our bench day by day can only further serve to increase our efficiency."
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Prof. Karl Mechtler

Head of Proteomics Tech Hub at VBCF, Vienna, Austria

Key benefits of BeatBox^®

Ensures reliable results
Excellent reproducibility, even for challenging samples
Scalability and flexibility combined
‍1-96 samples, compatible with both 96 well plates and single tubes
Suitable for any laboratory
Compact and quiet instrument for beginners and experts alike
Straightforward FFPE sample preparation
Xylene-free approach without separate deparaffinization step
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