Customer Statements

“As a core facility we often receive samples of unknown quality. To ensure the contaminants in these samples don’t end up clogging our precious equipment and leading to extensive down time we needed to find a reliable and robust workflow we could trust. The iST sample preparation workflow from PreOmics is our trusted option for global sample preparation. The kit produces clean desalted samples which absolutely decrease downtime of our instruments. The kit is even suitable for in-experienced users who may have never done a tryptic digest before. We are really happy with the ease-of-use and reproducibility and are recommending the PreOmics’ kit to our core facility colleagues and collaborators.”

— Karl Mechtler, Head of Core Facility at IMP, IMBA and GMI, Head of Mass Spectrometry VBCF

“While we were looking for a simpler sample preparation protocol, we tested PreOmics’ iST kit and were amazed by the results. The number of identified peptides & proteins was 20–30% higher compared to what is achieved using our standard lab procedure. On top of that, the iST kit and protocol are so convenient as it includes everything ready-to-use and allows you to save a lot of time. Using the iST kit enables you to start processing your samples in the morning and have them ready for LC-MS in the afternoon.”

— Dr. Manuel Tzouros (project leader), Discovery Proteomics, Roche Innovation Center Basel

“We recently used PreOmics’ sample preparation method to process mammalian cells in comparison to our standard laboratory methods. Using this new sample preparation technology we increased the efficiency of the tryptic peptide cleavage and obtained better identification rates in our samples. In addition, the short hands-on-time for the entire workflow as well as the comparatively low amounts of starting material now enable us to process more and be more time-efficient. We believe in this new method and will subsequently implement it in our future project designs.”

— Dr. Andreas Tebbe (Head of Mass Spectrometry), Evotec, Munich

“In the Core Facility Proteomics, we recently used the new PreOmics sample preparation kit to process blood plasma or serum samples. The kit drastically reduced the hands-on-time as well as the necessary amounts of starting material in comparison to standard protocols. We appreciate the high reproducibility and easy applicability of this kit for experienced as well as inexperienced users. As soon as available, we intend to buy PreOmics’ sample preparation kit.”

— Dr. Christine von Törne (Staff Scientist), Core-Facility Proteomics Helmholtz Center Munich

“In the Core Facility Proteomics of the LMU Munich, we have used the PreOmics’ sample preparation kit to process different kinds of samples from our users. We are really happy with the results and we have already recommended the PreOmics’ kit to our colleagues.”

— Dr. Ignasi Forné (Technical Director), Protein Analysis Unit, Biomedical Center Munich

“We recently worked with Dr. Garwin Pichler and Dr. Nils Kulak, the founders of PreOmics, to use MS-based proteomics to determine changes in expression profiles in monocyte-derived hepatocyte like cells, so-called MH cells or MetaHeps®. The impressive advantages of PreOmics’ sample preparation method, namely the comparatively low sample consumption as well as the reproducible sample processing, which are very important for patient- derived cells, enabled the quantitative measurement and analysis of protein expression profiles across different patients. Generally, we find very good consistency between quantitative MS- based measurements and results stemming from our assays on individual drug-hepatotoxicity. Moreover, the deep knowledge of Dr. Pichler and Dr. Kulak allowed to optimize the project design for MS-based proteomics. Their great experience helped with data analysis of the samples investigated. We appreciate the competence of the PreOmics team providing valuable user support.”

— Dr. Andreas Benesic (CEO), Metaheps, Munich

“We recently used PreOmics’ sample preparation kit and it worked perfectly. We obtained a higher number of peptide identifications (50–70% increase) in comparison to our standard laboratory methods. In addition, the peptides were extremely clean after digestion and purification. The complete workflow is very intuitive and the color-coding of the buffers is an excellent idea.”

— Dr. Marieluise Kirchner (Head), Core-Facility Proteomics Max-Delbrück-Centrum, Berlin

“We recently used PreOmics’ Kit for sample preparation of hematopoietic stem cells followed by nanoUHPLC MS analysis in direct comparison to iST that represents our standard laboratory method for “one pot” whole cell lysis, digestion and peptide clean-up. Our results clearly indicate that the PreOmics' kit outperformed our standard method in terms of both identification and quantification rates of proteins as well as peptides. In addition, we are convinced by the high reproducibility and efficiency of the approach. The complete protocol was fast, very easy to follow and the identification rates and sample quality exceeded our expectation!”

— Dr. Yaarub R. Musa & Dr. Gerhard Mittler, Max Planck Institute of Immunobiology and Epigenetics, Proteomics Research Facility, Freiburg

“Many of our clinical samples are rare specimens with limited tissue and protein yields that have to be divided not only for proteomics but also histological analysis and molecular biology studies. When you have a single, low-input shot for proteomics you can’t risk introducing the usual sources of variability that arise from typical proteolysis methods. In these situations, our researchers happily rely on the PreOmics iST kit knowing that they will have stabilized the sources of technical variations, permitting them to focus on the biology of our precious samples. The PreOmics team has worked closely with our researchers to consult with us on our unique biological questions and concerns. As a testament to its value, the PreOmics kits’ components are now integrated scientific lexicon and processing protocols, alongside traditional proteomics methods.”

— Dr. Sasha A. Singh, Director, Proteomics Research, Center for Interdisciplinary Cardiovascular Sciences, Division of Cardiovascular Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA.

“We at the clinic of the LMU Munich collaborate with the company Thermosome to study thermosensitive liposomes. I performed recently my very first proteomic sample preparation using the PreOmics kit. The kit was very intuitive, the protocol was very easy to follow and we got remarkable results. We definitely will order again.”